Central sensitization to pain appears to leave processing of C-Fiber input (e.g. heat pain thresholds) unchanged – thus, sensitization to heat pain is considered as a clear sign of peripheral sensitization. Unlike pain, less is known about the exact mechanisms of itch perception and sensitization phenomena. So far, it is assumed that pruritus sensitization is (similar to pain) mediated by A-beta fibers. However, since painful stimuli on eczema of patients with atopic dermatitis trigger itching, we have addressed the question of the role of C-fibers in this process. After sensitization with cowhage or histamine, itch and pain perception of electrical C-fiber stimuli were assessed on the itchy site and the surrounding area. A brush and a von-Frey Filament served as comparison. Our data show for the first time that peripheral pruritic activity causes spinal sensitization not only to A-fiber (alloknesis and hyperknesis), but also to C-fiber input. This indicates that spinal sensitization pattern for pain and itch are different. We propose that such  central sensitization for itch will also contribute to clinical itch conditions and represents a therapeutic target.