Dopamine (DA) is a robust neuromodulator of pain circuits in the anterior cingulate cortex (ACC). However, the impact of neuropathic pain on DA release and DA-sensitive circuits has not been investigated. Taking advantage of electrophysiology and in vivo fiber photometry, we demonstrate that neuropathic pain significantly affects DA release and DA-sensitive neurons in the ACC, promoting allodynia and hyperalgesia. Furthermore, since VTA DAergic projections to the ACC are VGluT2+, we explored whether glutamatergic signaling from these projections plays a role in pain perception.